Allen Institute for Brain Science

Why does it seem like some datasets are repeated across multiple rows?

You are correct that this is the case, and we understand it is not intuitive.

Is there a way to determine the molecular identity of the Observed Labeled Cell Population?

Yes. For all transgenic mouse lines and a subset of enhancer AAVs, we performed single cell RNA sequencing of the labeled cells to determine their molecular identity more accurately.

Are enhancer AAVs strong enough to drive expression of functional cassettes?

‍Previously published work by the Allen Institute and other labs has shown that enhancer AAVs are capable of driving sufficiently high levels of expression for functional cargo. However, this will depend on many parameters, such as the identity of the enhancer, the viral serotype, the delivery route, etc. Therefore, this property will need to be empirically evaluated for each enhancer before it is used.

Why do I sometimes see a fluorescent signal in an unexpected channel?

‍During image conversion for GTA, all image sets underwent an automatic optimization process for enhancement of brightness and contrast across all channels. Images with extremely bright fluorescence (to the point of saturation) in one of the channels, showed bleed-through into a neighboring channel.

How can I adjust the image settings or take snapshots of them?

‍Clicking on a specific row in the results table will enable a preview of the image set. For additional image options, you can click on the button above the preview image to access Neuroglancer.